Volume 2, Issue 2, May 2017, Page: 75-82
Enumeration of Bacteriophages from Mmabatho Treatment Plant and Some Selected Water Sources in Ngaka Modiri Molema District of North-West Province, South Africa
Theodora Ijeoma Ekwomadu, Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng, South Africa
Nomathamsanqa Patricia Sithebe, Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng, South Africa
Received: Nov. 1, 2016;       Accepted: Dec. 28, 2016;       Published: Jan. 20, 2017
DOI: 10.11648/j.ijmb.20170202.14      View  3042      Downloads  80
Abstract
Bacteriophages are viruses that infect bacteria, those that specifically infect E. coli and other coliforms are termed coliphages. While not a threat to humans, occurrence of somatic and F-RNA coliphages has been correlated with the presence of human enteric viruses in faecal-polluted water environments. This study focused on investigating the incidence of somatic coliphages and F-RNA coliphages from Mmabatho treatment plant and some selected water sources in Ngaka Modiri Molema District of the North West province. A total of 17 water samples were analysed for the presence of somatic and F-RNA phages using the double-agar-layer plaque assay according to the standard ISO method, ISO 10705-1(1995, 2000). The physico-chemical properties of the water samples were measured before sample collection. Bottled water was used as a negative control and the phage strains фX174 and MS2 as positive controls. Out of the 17 samples collected, (76%) were positive for somatic coliphages, no F-RNA coliphages were detected while 24% of the samples had no coliphages. Thus, the presence of coliphages indicates that the water sources do not meet up to national and international requirement for drinking water quality. Furthermore, monitoring water environments for possible faecal contamination is necessary to ensure public health and safety.
Keywords
Bacteriophages, Coliphages, Somatic, F-RNA, E. Coli, Dams, Ground Water Taps, Sewage Treatment Plant
To cite this article
Theodora Ijeoma Ekwomadu, Nomathamsanqa Patricia Sithebe, Enumeration of Bacteriophages from Mmabatho Treatment Plant and Some Selected Water Sources in Ngaka Modiri Molema District of North-West Province, South Africa, International Journal of Microbiology and Biotechnology. Vol. 2, No. 2, 2017, pp. 75-82. doi: 10.11648/j.ijmb.20170202.14
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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