Volume 3, Issue 2, June 2018, Page: 44-50
Antimicrobial Activities of Four Strains of Streptomyces sp Isolated from the Pond of the Village of Demba Tiarki Tara in Mali
Mamadou Wéléba Bagayoko, Department of Biology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali
Amadou Hamadoun Babana, Department of Biology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali
Adounigna Kassogué, Department of Biology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali
Doulaye Dembélé, Microarray and Sequencing Platform, Institute of Genetics and Molecular and Cellular Biology (IGBMC), University of Strasbourg, Illkirch, France
Received: Jun. 8, 2018;       Accepted: Jul. 4, 2018;       Published: Aug. 7, 2018
DOI: 10.11648/j.ijmb.20180302.13      View  415      Downloads  49
Abstract
The search for new molecules is needed to cope with the recrudescence of bacterial resistance. Actinomycetes, especially the genus Streptomyces remains the most requested for the production of bioactive substances. Nineteen samples of soil and mud from the pond of Demba Tiarki Tara were collected and treated. In total, 7 strains of Streptomyces were isolated by the calcium carbonate enrichment technique for the isolation of Streptomyces. The antimicrobial potency of isolated Streptomyces was assessed on human and plants pathogens (Staphylococcus aureus, Aspergillus flavus, Penicillium sp, Xanthomonas sp, Salmonella thyphi, Helminthos sp). Four of the tested actinmycetes strains showed antimicrobial activities against the pathogens used in the in vitro test. But only one strain showed high antagonist activity against Staphylococcus aureus and has a strong ability to produce melanin. Three strains were weakly active on Salmonella typhi. This result showed, for the first time, that the pond of Demba Tiarki Tara (DTT) contains bacteria producing bioactive compounds indicating the medical interest that the local population has in this pond. Secondary metabolite production by one strain may be an effective antibiotic for the management of resistant Staphylococcus aureus strains.
Keywords
Antimicrobial Activities, Streptomyces, Pond
To cite this article
Mamadou Wéléba Bagayoko, Amadou Hamadoun Babana, Adounigna Kassogué, Doulaye Dembélé, Antimicrobial Activities of Four Strains of Streptomyces sp Isolated from the Pond of the Village of Demba Tiarki Tara in Mali, International Journal of Microbiology and Biotechnology. Vol. 3, No. 2, 2018, pp. 44-50. doi: 10.11648/j.ijmb.20180302.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Baltz. RH (2008), Current Opinion in Pharmacology. Vol-8, issue 5, pg 557-563.
[2]
Pandey B, Ghimirel P, Prasard V, Thomas M, Chan Y, Ozanick S (2002). Studies of the antimicrobial activity of the actinomycetes isolated from the Khumby region of Nepal (Doctoral Thesis). Department of Bacteriology, university of Wisconsin-Madison, Madison, (Available at http://grad.uprm.edu/tesis/riverarivera.pdf).
[3]
Mims C, Dockrell HM, Goeringn RV, Roitt I, Wakelin D, Zuckerman M (2004). Attacking the enemy: Antimicrobial Agents and Chemotherapy: Macrolides. In: Medical Microbiology 3rd edn. London: Mosby Ltd; p. 489.
[4]
Oskay M, Tamer AU, Azeri C (2004). Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. Afr J Biotecnol; 3:441-446.
[5]
Woodford N, Turton JF, Livermore DM, (2011). Multi resistant Gram negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance. FEMS Microbiol Rev 35:736-755.
[6]
So AD, Gupta N, Brahmachari SK, Chopra I, Munos B, Nathan Outterson CK, Paccaud JP, Payne DJ, peeling RW, Spigelman M, Weigelt J (2011) Towards new business models for R&D for novel antibiotics. Drug Resist Updat 14:88-94.
[7]
Tiwari, K & Gupta, RK (2011), Critical Reviews in Biotechnology (doi:10.3109/07388551.2011.562482).
[8]
Watve MG, Tickoo R, Jog MD, Bhole BD (2001) How many antibiotics are produced by the genus Streptomyces? Arch Microbiol 176: 386-390.
[9]
Ferial, MR., Hayam, MF., Ayatollah, S. E. Z., Ahlam M. E. (2015), Isolation and characterization of multifunctional Streptomyces species with antimicrobial, nematicidal and phytohormones activities from marine environments in Egypt. Microbiological Research, 175 (2015) 34-47, http://dx.doi.org/10.1016/j.micres.2015.03.002.
[10]
Jose PA, Jebakumar SRD (2014) Unexplored hyper saline habitats are sources of novel actinomycetes. Front Microbiol 5:1-3.
[11]
Ganesan G, Velayudhan SS, Solomon Robinson DJ (2014) Antimicrobial potential of phylogenetically unique actinomycetes, Streptomyces sp. JRG-04 from marine origin. Biologicals 42:118-311.
[12]
Lee J. Y and Hwang B. K (2002). Diversity actinomycetes in various vegetative soils of Korea. Can. J. Microbiol. 48.407-417.
[13]
EL-Nakeeb, M. A. & Lechevalier, H. A. (1963). Selective isolation of aerobic actinomycetes. Appl. Microbiol. 11, 75-77.
[14]
Awad M., Elshahed K., Elnakkadi A. (2009) Isolation screening and identification of newly isolated soil Streptomyces (Streptomyces sp. NRC-35) for β-lactamase inhibitor production. Word Appl Sci J. 7 (5): 637-646.
[15]
Stackebrandt E, Rainey F. A and Ward-Rainey N. L. (1997) Proposal for a new hierarchic classification system Actinobacteria classic nov. Int J Syst Bacteriol. 47:479-491.
[16]
Fenical, W and Jensen PR. 1993. Marine microorganisms: a new biomedical resource. Marine Biotechnology. Volume 1, Pharmaceutical and Bioactive Natural Products. 1 (Attaway DH, Zaborsky OR, Eds.): 419-459., New York; London: Plenum Press Abstract.
[17]
Thumar, JT & Singh SP (2007), Brazilian Journal of Microbiology. Vol-33, issue 3, pg 210-215.
[18]
Charu S, Ramendra S. P., Pragya J and Ajay. K. (2017) Optimization of cultural condition for production of antifungal bioactive metabolite by Streptomyces spp. isolated from soil. Int. J. Curr. Microbiol. App. Sc (2017) 6 (2): 386-396. doi: http://dx.doi.org./10.20546/ijcmas.2017.602.043.
[19]
Lindholm P., Kortemaa H., Kokkola M., Haahtela K., Salonen M. S., and. Valkonen J. P. T. (1997) Streptomyces spp. Isolated from Potato Scab Lesions Under Nordic Conditions in Finland. November 1997, Volume 81, Number 11. 1317-1322. APS Journals https://doi.org/10.1094/PDIS.1997.81.11.1317.
[20]
Katz E, Thompson CJ, Hopwood DA. (1983). Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol 129: 2703-2714 DOI: 10.1099/00221287-129-9-2703.
[21]
Huber M, Hintermann G, and Lerch K, (1985). Primary structure of tyrosinase from Streptomyces glaucescens. Biochemistry 24 (22), pp 6038–6044. DOI: 10.1021/bi00343a003, ACS Legacy Archive.
[22]
Khattab A. I., Eltahir H., Babiker., Humodi A. S. (2016). Streptomyces: isolation, optimization of culture conditions and extraction of secondary metabolites. Int. Curr. Pharml. J. 5: 27-32.
[23]
Bundale S., Deovrat B., Nashikhar N., Kadam T., Upadhyay A. (2015). Optimization of culture conditions for production of bioactive metabolites by Streptomyces spp. Isolated from soil. Adv. Microbial., 5:441-451.
[24]
Nascimento R. P., Coelho RRR., Marques S., Alves L., Girio F. M., Bon EPS Amaral-Collaço MT. (2002). Production and partial characterization of xylanase from Streptomyces sp. Strain AMT-3 isolated from Brazilian cerrado soil. Enzyme Microb Technol. 31:549-555.
[25]
Mobeen S., G. Girija Sankar., M. Iswarya., P. Rajitha. (2017). Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10. Journal of Genetic Engineering and Biotchnology (2017) 15. 87-94.
[26]
Hasavada S. H., Thumar J. T., Singh S. P. (2006) Secretion of a potent antibiotic by salt tolerant and alkaliphic actinomyces Streptomyces annanensis strain RJT-1, Current Science. 91 (10): 1393-1397.
[27]
Atta H. M. (2009) An Antifungal Agent Produced by Streptomyces olivaceiscleroticus, AZ-SH514. Word Appl Sci J. 6 (11):1495-1505.
Browse journals by subject