Volume 3, Issue 3, September 2018, Page: 79-82
Detection of invA Gene in Salmonella Limete Isolated from Wastewater Treatment Plant of University of Nigeria Nsukka, Nigeria
Anthony Chibuogwu Ike, Department of Microbiology, University of Nigeria, Nsukka, Nigeria
Dickson Ihenrochi Dickson, Department of Microbiology, University of Nigeria, Nsukka, Nigeria
Okechukwu John Obi, Department of Microbiology, University of Nigeria, Nsukka, Nigeria
Received: Oct. 1, 2018;       Accepted: Oct. 22, 2018;       Published: Nov. 10, 2018
DOI: 10.11648/j.ijmb.20180303.13      View  141      Downloads  33
Abstract
Salmonellae are ubiquitous microorganisms that infect both humans and animals. Human infections usually occur through contaminated food or water and can result in one of two major diseases, namely gastroenteritis and enteric fever. Hence, Salmonella remains a major public health problem especially in developing countries where the level of hygiene is very low. The objective of this study was to evaluate the potential risk of Salmonella serovars isolated from the University of Nigeria Nsukka (UNN) wastewater treatment plants. Three Salmonella enterica subspecies enterica serovar Limete isolates from the UNN waste treatment plants were investigated for the presence of invasive A (invA) gene. Deoxyribonucleic acid (DNA) was extracted from the isolates by boiling method. Extracted bacteria DNA was amplified by polymerase chain reaction (PCR) using invA specific primers. PCR products were resolved on 1.5% agarose gel stained with 0.5 µg/ml of ethidium bromide. Results showed the presence of a band size of 244 base pair of Salmonella invA gene in 2 of the isolates. This is an indication that the isolates may have a human or animal origin and are potentially pathogenic. Therefore, the treatment of water in the wastewater plant is insufficient and water from the plant should not be employed for human use or used with caution.
Keywords
Salmonella Enterica, InvA Gene, Wastewater, DNA, PCR, Serovar
To cite this article
Anthony Chibuogwu Ike, Dickson Ihenrochi Dickson, Okechukwu John Obi, Detection of invA Gene in Salmonella Limete Isolated from Wastewater Treatment Plant of University of Nigeria Nsukka, Nigeria, International Journal of Microbiology and Biotechnology. Vol. 3, No. 3, 2018, pp. 79-82. doi: 10.11648/j.ijmb.20180303.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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