Volume 4, Issue 1, March 2019, Page: 19-23
New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans
Zenab Abduhall Krema, Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya
Ebtesam Salem Mokthar, Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya
Hameda Sadkali El Magrahi, Department of Medical Laboratory Science, Faculty of Medical Technology, Tripoli, Libya
Mohamed Suliman Ellabib, Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya
Received: Mar. 5, 2019;       Accepted: Apr. 8, 2019;       Published: May 15, 2019
DOI: 10.11648/j.ijmb.20190401.14      View  116      Downloads  20
Abstract
Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.
Keywords
Candida albicans, C. dubliniensis, Chlamydospore Production, Differential Medium, Seeds, Leaves
To cite this article
Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib, New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans, International Journal of Microbiology and Biotechnology. Vol. 4, No. 1, 2019, pp. 19-23. doi: 10.11648/j.ijmb.20190401.14
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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