Volume 4, Issue 3, September 2019, Page: 55-63
Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm
Pierrette Ngo Bahebeck, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon; Institute of Agricultural Research for Development (IRAD), Yaounde, Cameroon
Claire Stephane Metsopkeng, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Joelle Signe MBiada, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Chrétien Lontsi Djimeli, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Antoine Tamsa Arfao, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon; Microbiology and Biotechnology Laboratory, Saint Jerome Polytechnic Institute, Saint Jerome Catholic University of Douala, Douala, Cameroon
Paul Alain Nana, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Olive Vivien Noah Ewoti, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Luciane Marlyse Moungang, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Genevieve Bricheux, Laboratory 'Microorganisms: Genome and Environment', Clermont Auvergne University, Aubière Cedex, France
Télesphore Sime-Ngando, Laboratory 'Microorganisms: Genome and Environment', Clermont Auvergne University, Aubière Cedex, France
Moïse Nola, Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde, Yaounde, Cameroon
Received: May 22, 2019;       Accepted: Jun. 28, 2019;       Published: Jul. 24, 2019
DOI: 10.11648/j.ijmb.20190403.11      View  108      Downloads  33
Abstract
The aim of this study was to determine the cultivability of E. faecalis and enteropathogenic E. coli (EPEC) under different light intensities in the presence of different concentrations of mercury chloride (HgCl2) and biodegradable organic compound (BOC). Studied light intensities were 0, 500, 1000, 1500 and 2000 lux while studied HgCl2 concentrations were 0.001, 0.01, 0.1 and 1μg/L. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The BOC used was glucose at concentrations of 0.001, 0.01, 0.1 and 1mg/L and at pH 7. The duration of incubation under light was 6 h. Results showed that E. faecalis and EPEC bacteria are inactivated by HgCl2 irrespective of studied concentration. The cells inhibition percentage (CIP) of E. faecalis varied from 94.46% to 99.53% at 0.001μg/L, from 94.77% to 99.55% at 0.01μg/L, from 94.92% to 99.57% at 0.1μg/L and from 96.97% to 99.77% at 1μg/L of HgCl2. For EPEC cells, the CIP fluctuated between 89.87% and 98.99%, between 90.67% and 99.14%, between 92.05% and 99.14% and 93.50% and 99.25% respectively in solutions containing 0.001, 0.01, 0.1 and 1µg/L of HgCl2. The highest abundance was observed under 1500 lux for E. faecalis and 500 lux for EPEC. Exposure to light seemed to intensify the toxic action of HgCl2 in the medium. Cells metabolism reactivations under 2000 lux for E. faecalis and 1000 lux for EPEC were nevertheless observed. The level of this photo-reactivation varies from one organism species to another. The oligotrophic nature of the medium could not allow studied the microorganisms to set up specific protection mechanism against HgCl2 and light.
Keywords
Enterococcus faecalis, Escherichia coli, Light Intensity, Mercury Chloride, Biodegradable Organic Compound, Aquatic Microcosm
To cite this article
Pierrette Ngo Bahebeck, Claire Stephane Metsopkeng, Joelle Signe MBiada, Chrétien Lontsi Djimeli, Antoine Tamsa Arfao, Paul Alain Nana, Olive Vivien Noah Ewoti, Luciane Marlyse Moungang, Genevieve Bricheux, Télesphore Sime-Ngando, Moïse Nola, Assessment of the Effect of Light, HgCl2 and Organic Compound on Enterococcus faecalis and Escherichia coli Cells Survive in Aquatic Microcosm, International Journal of Microbiology and Biotechnology. Vol. 4, No. 3, 2019, pp. 55-63. doi: 10.11648/j.ijmb.20190403.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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