Volume 4, Issue 3, September 2019, Page: 64-71
Prevalence and Antimicrobial Resistance of Salmonella enterica Isolated from Chicken and Guinea Fowl in Burkina Faso
Soutongnooma Caroline Bouda, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso; National Agency for Food, Environment and Work Security (ANSES), Ploufragan-Plouzané, France
Assèta Kagambèga, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso; Departement of Biology, Institute of Sciences (IDS), Ouagadougou, Burkina Faso
Laetitia Bonifait, National Agency for Food, Environment and Work Security (ANSES), Ploufragan-Plouzané, France
Françoise Le Gall, National Agency for Food, Environment and Work Security (ANSES), Ploufragan-Plouzané, France
Hadiza Bawa Ibrahim, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso
Evariste Bako, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso
Toudwensida Serge Bagre, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso
Cheickna Zongo, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso
Aïssata Wereme N’diaye, National Institute for Environment and Agricultural Research CNRST / INERA / CREAF, Ouagadougou, Burkina Faso
Sababenejo Alfred Traore, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso
Marianne Chemaly, National Agency for Food, Environment and Work Security (ANSES), Ploufragan-Plouzané, France
Gilles Salvat, National Agency for Food, Environment and Work Security (ANSES), Ploufragan-Plouzané, France
Nicolas Barro, Department of Biochemistry and Microbiology, University of Ouaga I, Ouagadougou, Burkina Faso
Received: Jun. 8, 2019;       Accepted: Jul. 12, 2019;       Published: Jul. 31, 2019
DOI: 10.11648/j.ijmb.20190403.12      View  155      Downloads  49
Abstract
Consumption of contaminated poultry and poultry products by Salmonella is a public health problem worldwide. The aim of this study was to determine the prevalence, serotypes and antibiotic susceptibility of Salmonella isolated from slaughtered poultry. A total of 563 intestine samples from slaughtered chickens (n= 283) and guinea fowl (n=280) were collected from 7 open markets in 2 urban and 2 rural areas of Burkina Faso. The samples were processed for the isolation and identification of associated Salmonella using microbiological standard methods. The suspected colonies were subjected to biochemical tests and serotyped by slide agglutination test according to Kauffman-White scheme. Antibiotic sensitivity patterns of Salmonella were also investigated using commonly used antibiotics. Of the 563 intestines samples, 139 (24.69%) isolates were recovered, with 59/283 (20.84%) isolates from chicken and 80/280 (28.57%) isolates from guinea fowl. The successful serotyping of 109/139 isolates revealed 9 serotypes namely Typhimurium, Kentucky, Ouakam, Brancaster, Hato, Kaapstad, Essen, Chester, and Derby. Five strains were untypable and 15 belong to different serogroups such as B, M, E, D, F, and O. The serotypes Brancaster, Chester, Derby, Hato, and Typhimurium were found in chicken and guinea fowl. S. Kaapstad was detected only on guinea fowl and S. Essen, Kentucky and Ouakam on chicken. Serotype Derby (38.84%), Chester (11.51%) and Hato (10.07%), Typhimurium (8.63%) were the most prevalent. Out of the 139 isolates, 100% show resistance to at least one antibiotic (Erythromycin), while, 50 (35.97%) were multi-resistant. High sensitivity of isolates was recorded for Chloramphenicol, Ciprofloxacin, Nalidixic acid, Cephalexin, Sulfamethoxazole–trimethoprim and Colistin Sulfate. The data confirm that poultry is a potential reservoir of Salmonella. This recommends good hygienic practices when producing poultry carcasses.
Keywords
Salmonella Serotypes, Antimicrobial Resistance, Chicken, Guinea Fowl, Burkina Faso
To cite this article
Soutongnooma Caroline Bouda, Assèta Kagambèga, Laetitia Bonifait, Françoise Le Gall, Hadiza Bawa Ibrahim, Evariste Bako, Toudwensida Serge Bagre, Cheickna Zongo, Aïssata Wereme N’diaye, Sababenejo Alfred Traore, Marianne Chemaly, Gilles Salvat, Nicolas Barro, Prevalence and Antimicrobial Resistance of Salmonella enterica Isolated from Chicken and Guinea Fowl in Burkina Faso, International Journal of Microbiology and Biotechnology. Vol. 4, No. 3, 2019, pp. 64-71. doi: 10.11648/j.ijmb.20190403.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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