Volume 4, Issue 3, September 2019, Page: 94-102
Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria
Nasir Tukur Dabo, Department of Biological Sciences, Bayero University Kano, Kano, Nigeria
Bashir Muhammad, Department of Microbiology, Bayero University Kano, Kano, Nigeria
Habeeb Kayode Saka, Department of Microbiology, Bayero University Kano, Kano, Nigeria; Department of Microbiology, Nigerian Stored Products Research Institute, Kano, Nigeria
Zaharaddin Muhammad Kalgo, Department of Microbiology, Federal University Birnin Kebbi, Kebbi State, Nigeria
Rasaki Adewole Raheem, Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Nigeria
Received: May 21, 2019;       Accepted: Jun. 25, 2019;       Published: Sep. 10, 2019
DOI: 10.11648/j.ijmb.20190403.15      View  104      Downloads  40
Abstract
Antibiotic resistance in bacterial pathogens is a global health challenge linked to high morbidity and mortality. This study was carried out among under-five children attending three major hospitals in Kano State namely: Murtala Muhammad Specialists Hospital (MMSH), Wudil General Hospital (WGH) and Bichi General Hospital (BGH), representing the three senatorial districts. Rectal swab specimens from 400 diarrhoeic and 50 non-diarrhoeic children were collected with a sterile transport swab containing Carry-Blair Medium. These were inoculated onto MacConckey and Salmonella-Shigella Agar and incubated at 37°C for 18-24 hours for isolation of bacteria. Bacterial isolates were subjected to battery of biochemical tests (IMViC and KIA) for the identification of Escherichia coli. Antimicrobial susceptibility test (AST) was carried out on E. coli isolated using modified Kirby-Bauer method. The findings revealed 74% and 66% E. coli recovery from case and control subjects respectively. The AST revealed trimethoprim-sulfamethoxazole 75.1% was the most resisted antibiotic, significantly different between the case subjects (P-value=0.031), 83% were resistant to at least one class of antibiotic, 44.4% resistant to two classes of antibiotics and significantly higher in the control group (P-value=0.006) and 8.0% MDR rate. Female subjects shows higher resistance to the tested antibiotics but the differences were only significant in amoxicillin-clavulanic acid (49.6% versus 32.9%; P-value=0.003) and trimethoprim-sulfamethoxazole (91.7% versus 57.1%; P-value=0.037) in the case and control group respectively. It can be concluded that E. coli is the predominant agent associated with diarrhoea in Kano children and high resistance of trimethoprim-sulfamethoxazole is noteworthy. Hence the need to revise the current diarrhoea treatment regimen for Kano children.
Keywords
Escherichia coli, Antibiogram, Diarrhoeic, Resistance, Kano
To cite this article
Nasir Tukur Dabo, Bashir Muhammad, Habeeb Kayode Saka, Zaharaddin Muhammad Kalgo, Rasaki Adewole Raheem, Antibiotic Resistance Pattern of Escherichia coli Isolated from Diarrhoeic and Non-diarrhoeic Under Five Children in Kano, Nigeria, International Journal of Microbiology and Biotechnology. Vol. 4, No. 3, 2019, pp. 94-102. doi: 10.11648/j.ijmb.20190403.15
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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