Volume 5, Issue 1, March 2020, Page: 16-21
Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp
Bryan Pillacela, Biology Research Center, Faculty of Marine Sciences, Santa Elena Peninsula State University, La Libertad, Ecuador
Janeth Galarza, Biology Research Center, Faculty of Marine Sciences, Santa Elena Peninsula State University, La Libertad, Ecuador
Carolina Tufiño, Biology Research Center, Faculty of Marine Sciences, Santa Elena Peninsula State University, La Libertad, Ecuador
Received: Dec. 22, 2019;       Accepted: Jan. 17, 2020;       Published: Jan. 31, 2020
DOI: 10.11648/j.ijmb.20200501.13      View  42      Downloads  48
Abstract
Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anti-carcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.
Keywords
In silico Model, Phytoene Desaturase, Restriction Enzymes, Bioinformatics, Haematococcus sp
To cite this article
Bryan Pillacela, Janeth Galarza, Carolina Tufiño, Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp, International Journal of Microbiology and Biotechnology. Vol. 5, No. 1, 2020, pp. 16-21. doi: 10.11648/j.ijmb.20200501.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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