Volume 5, Issue 1, March 2020, Page: 22-27
Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis
James Hamuel Doughari, Department of Microbiology, School of Life Sciences, Moddibo Adama University Technology, Yola, Nigeria
Musa Jamila Bazza, Department of Microbiology, School of Life Sciences, Moddibo Adama University Technology, Yola, Nigeria
Received: Jan. 10, 2020;       Accepted: Jan. 27, 2020;       Published: Feb. 19, 2020
DOI: 10.11648/j.ijmb.20200501.14      View  83      Downloads  77
Limonene is essential oil isolated from pericarp of C. sinensis it is well known for its antimicrobial and antiseptic activities. Limonene has many medicinal properties and is widely used for the treatment of stomach aches, breast cancer, and as tonic for the digestive system, immune system and skin. It is also used to treat and prevent vitamin deficiencies, colds, flu, and typhoid fever, and to help fight viral and bacterial infections. The aim of this work was to determine the composition, antioxidant and antibacterial properties of limonene from pericarp of C. sinensis. Limonene from C. sinensis pericarps were extracted using steam distillation method. Phytochemical screening of the extracts revealed the presence of terpenoids and limonene in the extracts while GC/MS analysis showed highest percentage of limonene (96%). Result of antioxidant activity determination showed that limonene demonstrated strong scavenging activity (92.42%). Antimicrobial activity of the limonene extracts was evaluated by agar well method against S. aureus, S. pyogenes, P. aeruginosa, S. typhi while MIC and MBC were determined using the agar well dilution technique. Result showed that limonene extract exhibited an MIC of 200 mg/ml and MBC range of 300-400 mg/ml against all the isolates. The antibacterial effects of the extracts suggest their possible inclusion as candidate agents for development of chemotherapeutic agents against infection caused by the bacterial pathogen s tested.
Limonene, GS-MS Analysis, Phytochemical, Antioxidant, Antibacterial Activity
To cite this article
James Hamuel Doughari, Musa Jamila Bazza, Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis, International Journal of Microbiology and Biotechnology. Vol. 5, No. 1, 2020, pp. 22-27. doi: 10.11648/j.ijmb.20200501.14
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