Volume 5, Issue 1, March 2020, Page: 28-33
Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State
Ringim Mahi Saidu, Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria
Gumel Ahmad Mohammed, Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria
Shiaka Gimba Peter, Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria
Received: Jan. 26, 2020;       Accepted: Feb. 12, 2020;       Published: Feb. 19, 2020
DOI: 10.11648/j.ijmb.20200501.15      View  83      Downloads  70
Abstract
The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.
Keywords
Bacteria, Bioremediation, Hydrocarbon, Oil Spill, Petroleum, Pollution, Polymerase Chain Reaction (PCR)
To cite this article
Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter, Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State, International Journal of Microbiology and Biotechnology. Vol. 5, No. 1, 2020, pp. 28-33. doi: 10.11648/j.ijmb.20200501.15
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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