Volume 5, Issue 3, September 2020, Page: 93-96
Evaporative Coolers Air Conditioners Devices as a Source of Legionella Pneumophila in Khartoum State, Sudan and Recommended Measures of Control
Reem Mohamed Elfatih Ali Elsanousi, Department of Microbiology, College of Medicine, University of Bahri, Khartoum, Sudan
Received: May 8, 2020;       Accepted: May 29, 2020;       Published: Jun. 9, 2020
DOI: 10.11648/j.ijmb.20200503.13      View  347      Downloads  106
Abstract
This study was designed to substantiate the role of the evaporative coolers air conditioners in the proliferation of the genus Legionella. In addition to sort out, isolate and identify the genus Legionella including Legionella pneumophila, the causative agent of Legionnaires' disease and Pontiac fever from a new source. This type of air conditioners depends on water in their operation. They cool the air by filtering it through evaporation of water which is widely used in the Sudan. The evaporative air conditioning units considered as reservoirs of the ubiquitous bacteria which it harbored by amoeba. Legionella species can proliferate in amoeba and in biofilms that promote the growth of the bacteria even in the bad conditions. The study was done in Khartoum State including different premises from which water samples of evaporative coolers air conditioners were collected. Five hundred and fifty five water samples were collected, treated, cultured, identified and confirmed by gold standard culture technique using Buffered Charcoal Yeast Extract Agar (BCYE - α medium), Glycine Vancomycin Polymxin-Cycloheximide Agar (G V P C Medium) and biochemical techniques well as Real Time PCR test. The results of Legionella species obtained from this study were 42.3% of the total collected samples with a rate of 11% for Legionella pneumophila. Recommended measures of control must be applied including this type of air conditioners to prevent Legionella out breaks.
Keywords
Evaporative Coolers Air Conditioners Devices, Legionella pneumophila, Control Measures
To cite this article
Reem Mohamed Elfatih Ali Elsanousi, Evaporative Coolers Air Conditioners Devices as a Source of Legionella Pneumophila in Khartoum State, Sudan and Recommended Measures of Control, International Journal of Microbiology and Biotechnology. Vol. 5, No. 3, 2020, pp. 93-96. doi: 10.11648/j.ijmb.20200503.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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