Volume 5, Issue 3, September 2020, Page: 103-109
Assessment of Haematological Parameters of Pulmonary Tuberculosis Patients with and Without HIV Infection Attending Two Secondary Health Facilities in Jigawa State
Baballe Abbas, Department of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, Nigeria
Musa Mustapha Dogara, Department of Biological Sciences, Federal University Dutse, Dutse, Jigawa State, Nigeria
Nura Muhammad Sani, Department of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, Nigeria
Ahmad Mohammed Gumel, Department of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, Nigeria
Danjuma Lawal, Department of Microbiology and Biotechnology, Federal University Dutse, Dutse, Jigawa State, Nigeria
Received: May 4, 2020;       Accepted: Jun. 15, 2020;       Published: Jun. 29, 2020
DOI: 10.11648/j.ijmb.20200503.15      View  87      Downloads  43
TB and HIV form a lethal combination, as each fuels the progress of the other in the infected patients. This study assessed the haematological parameters of pulmonary tuberculosis (PTB) patients with and without HIV infection attending the two secondary health facilities in Jigawa State, Nigeria. A total of 150 PTB patients visiting the two secondary health facilities were randomly selected for the study. 5ml of venous blood was drawn aseptically with the help of sterile syringe using vein puncture technique and transferred into EDTA bottle to avoid coagulation. 2ml of the EDTA blood was transferred in to plane test tube for the haematological analysis using automated Haematological Analyser (Abacus Junior 380) while the remaining 3ml of the blood was placed into a Western green tube up to the zero mark for Erythrocyte sedimentation rate (ESR) determination. The data was analysed using SPSS version 20, One-way ANOVA was used to check the significant difference among the new, follow-up and Multi drug resistant TB (MDR-TB) groups of patients. 136 (90.67%) patients were HIV negative and 14 (9.33%) were HIV positive, 95 (63.33%) were males and 55 (36.37%) were females. Among the studied population 56 (37.33%) and 94 (62.67%) were new and follow-up PTB patients respectively. In this study, there were significantly lower mean values at (P>0.05) of White blood cells, lymphocytes, neutrophils (MID), granulocytes, Red Blood Cells, packed cells volume, and Platelets counts among PTB-HIV co-infected patients when compared with PTB patients. Of the PTB patients, 27.94% were Leukopenic, 25.7% Lymphopenic, 26.47% neutropenic, 30.88% anaemic and 20.59% thrombocytopenic. On the other hand, of the PTB-HIV co-infected patients, 64.29% were leukopenic, 35.71% lymphopenic, 26.47% neutropenic, 21.43% anaemic, and 14.29% thrombocytopenic. This study demonstrated high prevalence of leucopenia, followed by neutropenia and anaemia, lymphopenia, and thrombocytopenia. There was also high ESR values among more than two-third of the 150 PTB patients. The study also revealed that, females were more co-infected with HIV 64.29% than the males with 35.71%. HIV co-infection worsens haematological abnormalities of PTB patients. Knowledge of these haematological parameters will enhance the overall management of the PTB patients with regard to monitoring the disease progression and response to antimicrobial chemotherapy as they will serve as useful indicators for treatment success or failure. It is recommended that all newly diagnosed and follow-up PTB and PTB-HIV co-infected patients should be exposed to haematological counts to monitor their immune status.
Pulmonary Tuberculosis, Human Immunodeficiency Virus, Haematological Parameters, New TB Patients, Follow up TB Patients, Erythrocyte Sedimentation Rate
To cite this article
Baballe Abbas, Musa Mustapha Dogara, Nura Muhammad Sani, Ahmad Mohammed Gumel, Danjuma Lawal, Assessment of Haematological Parameters of Pulmonary Tuberculosis Patients with and Without HIV Infection Attending Two Secondary Health Facilities in Jigawa State, International Journal of Microbiology and Biotechnology. Vol. 5, No. 3, 2020, pp. 103-109. doi: 10.11648/j.ijmb.20200503.15
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cole S. T, Brosch R, and Parkhil J, Garnier T, Chucher C, Harris D, and Barrell B. G, (1998). “Deciphering the biology of Mycobacterium tuberculosis from the complete genome Sequence”. Pub Med Central. PMID 9634225.
Global tuberculosis report 2019. Geneva: World Health Organization; 2019. Licence: CCBY-NC-SA 3.0 IGO. ISBN 978-92-4-156571-4.
Lawanson A, and Emperor U, (2019). “Nigeria to expand diagnosis and treatment of tuberculosis”, NTBLCP 2019 pre-world tuberculosis day press briefing. Abuja. The Nation News Paper.
WHO (2017). “HIV/AIDS Fact Sheet.” Geneva: WHO Media Centre, updated July 2017.
NACA (2017), “National Strategic Framework on HIV and AIDS, 2017 -2021.” Abuja, National Agency for the Control of AIDS.
Global Funds to fight AIDS, Tuberculosis & Malaria, 2017, Geneva, Switzerland, website@theglobalfund.org.
Amilo G. I, Meludiu S. C, Ele P. U, Ezechukwu C, Onyenekwe C, and Ifeanyi C. M, (2013), Haematologic Indices in pulmonary Tuberculosis with and without HIV Co-infection in South Eastern Nigeria, Advances in Life Sciences and Technology, vol. II, 2013.
Hoffbrand A. V, and Petit J. E, (2005). “Clinical Haematology”, 3rd Edition. Mosby, London, Wiley & Sons Ltd Publishers.
Faven A, Aregawi Y, Agumas S, and Bamlaku E, (2018). “Haematological Abnormalities of pulmonary tuberculosis patients with and without HIV at the University of Gondar Hospital, Northwest Ethiopia”: A comparative cross-sectional studies”. Tuberculosis Research and Treatment Journal, Volume 2018.
NPC, and NBS, (2017). “National Population Commission of Nigeria and National Bureau of Statistics.” Abuja. www.nigerianstat.gov.ng.
Sei W. L, Young A. K, Young S. Y, Sang-Won U, Sang M. L, Chul-Gyu Y, Young W. K, Sung K. H, Young-Soo S, and Jae-Joon Y. (2006). “The prevalence and evolution of anemia associated with tuberculosis,” Journal of Korean Medical Science, vol. 21, no. 6, pp. 1028–1032, 2006. PMID: 1717968.
Tabarsi P, Mirsaeidi S. M, Amiri M, Mansouri S. D, Masjedi M. R, and Velayati A. A, (2008). “Clinical and laboratory profile of patientswith tuberculosis/HIVcoinfection at a national referral centre: Acase series,” EasternMediterraneanHealth Journal, Vol. 14, no. 2, pp. 283–291.
Yaranal P. J, Umashankar T, and Harish S. G, (2013) “Hematological profile in pulmonary tuberculosis,” International Journal of Health and Rehabilitation Sciences, vol. 2, no. 1, pp. 50–55.
Mohammed Abaker Saeed, (2013). “Measurement of Some Haematological parameters among Patients with pulmonary Tuberculosis in Khartoum State, Sudan”. University of Science and Technology, College of Medical Laboratory Science, 2013.-52p.: ill.; 28cm.-M. sc. http://repository.sustech.edu/handle/123456789/3503.
Isanaka S, Mugusi F, Urassa W, Willett W. C, Bosch R. J, Villamor E, Spiegelman D, Duggan C, and Fawzi W. W, (2012), “Iron deficiency and anemia predict mortality in patients with tuberculosis,” Journal of Nutrition, vol. 142, no. 2, pp. 350–357, 2012.
Tasnuva, T. and Sanjida, H. T., (2013). “An Evaluation of Sputum smear conversion and Haematological parameter alteration in early detection period of new pulmonary tuberculosi (PTB) patients”. International Journal of Medicine, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. Vol. 7, No. 1, 2013.
Baynes R. D., Flax H., Bothwell T. H, Bezwoda W. R, MacPhall A. P, Atkinson P, and Lewis D, (1986). “Haematological and Iron-related measurements in active pulmonary tuberculosis.” European Journal of Haematology, Vol. 36, no. 3, pp. 280–287.
Singh P. P, and Goyal A, (2013). Interleukin-6: a potent biomarker of Mycobacterial infection, Springer plus, vol. 2, Article 686.
Shafee M, Abbas F, Ashraf M, Mengal M. A, Kakar N, Ahmad Z and Ali F, (2014). “Hematological profile and risk factors associated with pulmonary tuberculosis patients in Quetta, Pakistan,” Pakistan Journal of Medical Sciences, vol. 30, no. 1, pp. 36–40, 2014.
Sharma S. K, Pande J. N, Singh Y. N, Verma K, Kathait S. S, Khare S. D, and Malaviya, (1992). “Pulmonary function and immunologic abnormalities in Miliary tuberculosis.” American Review of Respiratory Disease, vol. 145, no. 5, pp. 1167–1171, 1992.
Al-muhammadi, M. O, and Al-Shammery H. G, (2011). “Studying Some Hematological Changes in Patients with Pulmonary Tuberculosis in Babylon Governorate,” Medical Journal of Babylon, Vol. 8, no. 4.
Shareef H. A, and Amin N. R. M, (2012). “Abnormalities of hematological parameters in newly diagnosed Pulmonary tuberculosis patients in Kirkuk city,” Journal of University of Babylon, vol. 20, no. 5, pp. 1486–1492, 2012.
Okafor, I. M., Eyo, A. O., Okon E. A. and Akpan, P. A. (2013). “Some Haematological parameters of Tuberculosis infected Nigerians: A case study in Calabar – Nigeria”. Global Journal of Pure and Applied sciences, Vol. 19, 2013 pp. 98 – 99.
Bozoky, G., Ruby, E., Goher, I., Toth, J. and Mohos, A. (1997). “Hematologic abnormalities in pulmonary tuberculosis”, Orvosi Hetilap, April, 1997, Vol. 138, issue 17, pp. 1053-1056.
Yan S. L. S, Russell J, and Granger D. N, (2014). “Platelet activation and platelet-leukocyte aggregation elicited in experimental colitis are mediated by interleukin-6.” Inflammatory Bowel Diseases, Vol. 20, no. 2, pp. 353–362.
Mugusi F. M, Mehta S, Villamor E, Willy U, Elmar S, Ronald J. B, and Wafaie W. F, (2009). “Factors associated with mortality in HIV-infected and uninfected patients with pulmonary tuberculosis,” BMCPublicHealth, vol. 9, no. 1, p. 409, 2009.
Panda S., Meher L. K., Dalai S. P., Nayak S., and Tripathy S. K, (2016). “A case of immune thrombocytopenic purpura secondary to pulmonary tuberculosis,” Journal of Clinical and Diagnostic Research, vol. 10, no. 10, pp. OD12–OD13, 2016.
Kaur, K. P., Arora, B., Chhina, D. K., and Gupta, V. (2012). “Comprehensive evaluation of patient characteristics and clinical parameters as a diagnostic aid in tuberculosis”. The International Journal of Laboratory Medicine, Vol. 5, no 1.
Talat, T., Bhatti, B. M., and Yaqoob, M. (2002). “Comparative efficacy of different laboratory techniques used in diagnosis of tuberculosis in human population”, Journal of Medical Sciences, 2002, Vol. 2, issue 3 pp. 137-44.
Mandal S. K, and Chavan L, (2016). “Erythrocyte Sedimentation Rate Values in Cases of Active Tuberculosis without HIV Co-Infection.” Journal of Medical Science and Clinical Research, vol. 04, no. 10, pp. 13156–13159, 2016.
Paton N. I, Ng Y, Chee C. B, Persaud C, and Jackson A. A, (2003). “Effects of tuberculosis and HIV infection on whole-body protein metabolism during feeding, measured by the [15N] glycine method,” American Journal of Clinical Nutrition, vol. 78, no. 2, pp. 319–325, 2003.
Willey J. M, Sherdhood L. M, and Woolveton C. J. (Eds) (2018). Mycobaterium tuberculosis infections. Prescott, Harley, and Klein’s Microbiology 7th edition, pp 951-955.
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