Actinomycetes are a filamentous bacterium that belongs to the phyla actinobacteria. They are one of the most attractive sources of antibiotics. In this study, Isolation of Actinomycetes strain was obtained by serial dilution method and grown on starch casine agar (SCA). Soil sample were collected from Addis Ababa Science and Technology University (AASTU) and tests were performed to assess the presence of Actinomycetes using macroscopic observation and microscopic methods. Potential actinomycete isolates were also identified as gram positive by using Gram staining technique. The five out of twelve isolates showed coiled and rod shaped spore resembling the genus Streptomycets and antibacterial activities were evaluated for their antibacterial activity. Three out of five potential actinomycete isolates (isolate number 2, 4, and 5) showed anti bacteria activity, of which one isolate (isolate number 5) showed good inhibitory result when tested against two strains of microorganisms (E.coli and S. aures). Isolate 5 was found to exhibit significant antibacterial activity against the gram positive bacteria, Staphylococcus aures. It is concluded that the biowaste soil sampled from AASTU campus harbors a variety of potential antibiotic producing Actinomycetes, which can be used up for further exploration.
| Published in | International Journal of Microbiology and Biotechnology (Volume 5, Issue 4) |
| DOI | 10.11648/j.ijmb.20200504.15 |
| Page(s) | 203-206 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Actinomycetes, Antibiotics, Streptomycets
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APA Style
Rahel Tilahun. (2020). Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples. International Journal of Microbiology and Biotechnology, 5(4), 203-206. https://doi.org/10.11648/j.ijmb.20200504.15
ACS Style
Rahel Tilahun. Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples. Int. J. Microbiol. Biotechnol. 2020, 5(4), 203-206. doi: 10.11648/j.ijmb.20200504.15
AMA Style
Rahel Tilahun. Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples. Int J Microbiol Biotechnol. 2020;5(4):203-206. doi: 10.11648/j.ijmb.20200504.15
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Download@article{10.11648/j.ijmb.20200504.15,
author = {Rahel Tilahun},
title = {Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples},
journal = {International Journal of Microbiology and Biotechnology},
volume = {5},
number = {4},
pages = {203-206},
doi = {10.11648/j.ijmb.20200504.15},
url = {https://doi.org/10.11648/j.ijmb.20200504.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200504.15},
abstract = {Actinomycetes are a filamentous bacterium that belongs to the phyla actinobacteria. They are one of the most attractive sources of antibiotics. In this study, Isolation of Actinomycetes strain was obtained by serial dilution method and grown on starch casine agar (SCA). Soil sample were collected from Addis Ababa Science and Technology University (AASTU) and tests were performed to assess the presence of Actinomycetes using macroscopic observation and microscopic methods. Potential actinomycete isolates were also identified as gram positive by using Gram staining technique. The five out of twelve isolates showed coiled and rod shaped spore resembling the genus Streptomycets and antibacterial activities were evaluated for their antibacterial activity. Three out of five potential actinomycete isolates (isolate number 2, 4, and 5) showed anti bacteria activity, of which one isolate (isolate number 5) showed good inhibitory result when tested against two strains of microorganisms (E.coli and S. aures). Isolate 5 was found to exhibit significant antibacterial activity against the gram positive bacteria, Staphylococcus aures. It is concluded that the biowaste soil sampled from AASTU campus harbors a variety of potential antibiotic producing Actinomycetes, which can be used up for further exploration.},
year = {2020}
}
TY - JOUR T1 - Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples AU - Rahel Tilahun Y1 - 2020/12/16 PY - 2020 N1 - https://doi.org/10.11648/j.ijmb.20200504.15 DO - 10.11648/j.ijmb.20200504.15 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 203 EP - 206 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20200504.15 AB - Actinomycetes are a filamentous bacterium that belongs to the phyla actinobacteria. They are one of the most attractive sources of antibiotics. In this study, Isolation of Actinomycetes strain was obtained by serial dilution method and grown on starch casine agar (SCA). Soil sample were collected from Addis Ababa Science and Technology University (AASTU) and tests were performed to assess the presence of Actinomycetes using macroscopic observation and microscopic methods. Potential actinomycete isolates were also identified as gram positive by using Gram staining technique. The five out of twelve isolates showed coiled and rod shaped spore resembling the genus Streptomycets and antibacterial activities were evaluated for their antibacterial activity. Three out of five potential actinomycete isolates (isolate number 2, 4, and 5) showed anti bacteria activity, of which one isolate (isolate number 5) showed good inhibitory result when tested against two strains of microorganisms (E.coli and S. aures). Isolate 5 was found to exhibit significant antibacterial activity against the gram positive bacteria, Staphylococcus aures. It is concluded that the biowaste soil sampled from AASTU campus harbors a variety of potential antibiotic producing Actinomycetes, which can be used up for further exploration. VL - 5 IS - 4 ER -
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