This study focuses on the isolation and characterization of microbes from landfill soil in Gorakhpur, India. Landfills are a major source of soil pollution, and understanding the microbial composition in these environments is crucial for assessing soil quality and developing remediation strategies. Microorganisms can degrade numerous of organic pollutants owing to their metabolic machinery and to their capacity to adapt to inhospitable environment. Thus, microorganisms are major players in site remediation. However, their efficiency depends on many factors, including the chemical nature and the concentration of pollutants, their availability to microorganisms, and the physio-chemical characteristics of the environment. The research identified a diverse microbial community in the landfill soil, with predominant bacterial representatives including Gamma-proteobacteria, firmicutes, and bacteroids. Gram staining revealed the prevalence of gram-positive bacilli, along with distinct fungal species. These findings emphasize the potential of microorganisms in degrading organic pollutants and transforming various compounds in landfill soil. By elucidating the microbial diversity in landfill sites, this study provides insights for sustainable waste management practices and environmental conservation efforts. One of the major cause of soil pollution are landfills. There are the sites designated for dumping rubbish, garbage, or other sorts of solid wastes. Because most of these waste materials are non-biodegradable, they heap in the landfills where they stay for years, impacting on soil quality and polluting the land. The aim of this study is to isolate and investigate the role of microorganisms in a particular landfill area of Gorakhpur, Uttar Pradesh and to identify the microbial community found in that particular area.
| Published in | International Journal of Microbiology and Biotechnology (Volume 9, Issue 3) |
| DOI | 10.11648/j.ijmb.20240903.13 |
| Page(s) | 61-67 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Isolation, Site Remediation, Landfill Sites, Pollutants, Microbial Community
No. of colonies | Colour | Texture |
|---|---|---|
Colony 1 | Orange | Moist |
Colony 2 | Diffused white | Mucoid |
Colony 3 | Pure white | Dry |
Colony 4 | Yellow | Moist |
S. No. | Colour of bacterial colony | Shape observed | Appearance | Gram's class |
|---|---|---|---|---|
1 | Orange | Bacilli | Purple | Gram positive |
2 | White | Irregular | Purple | Gram positive |
3 | Diffused white | Spherical | Purple | Gram positive |
4 | Yellow | Irregular | Purple | Gram positive |
S. No. | Selected antibiotics | Antibiotic conc. | Bacterial culture conc. | Zone of inhibition |
|---|---|---|---|---|
1. | Streptomycin | 5mg/ml | 0.1ml | 2.6cm |
2. | Cefotaxime | 5mg/ml | 0.1ml | 3.5cm |
3. | Ceftriaxone | 5mg/ml | 0.1ml | 3.1cm |
4. | Distilled water | ______ | 0.1ml | 0.0cm |
S. no. | Selected antibiotics | Antibiotic conc. | Bacterial culture conc. | Zone of inhibition |
|---|---|---|---|---|
1. | Cefotaxime | 5mg/ml | 0.1ml | 3.50cm |
2. | Cefotaxime | 2.5mg/ml | 0.1ml | 3.30cm |
3. | Cefotaxime | 1.25mg/ml | 0.1ml | 2.90cm |
4. | Distilled water | _______ | 0.1ml | 0.00cm |
S. no. | Selected antibiotics | Antibiotic conc. | Bacterial culture conc. | Zone of inhibition |
|---|---|---|---|---|
1. | Streptomycin | 5mg/ml | 0.1ml | 3.5cm |
2. | Cefotaxime | 2.5mg/ml | 0.1ml | 3.2cm |
3. | Distilled water | ______ | 0.1ml | 0.0cm |
PAH | Polyaromatic Hydrocarbons |
DNA | Deoxyribonucleic Acid |
PDA | Potato Dextrose Agar |
LB | Luria Bertani |
| [1] | Mohd Arshad Siddiqqui (2017). Molecular characterization of bacterial species isolated from landfill site. Research Journal Environment Science 11(2): 65-70 |
| [2] | Jackie Aislabie, Julie R. Deslippe (2013). Soil microbes and their contribution to soil services. Soil microbial diversity 1.12. |
| [3] | Obire, O., Nwaubeta, O., Adue, S. B. N. (2002). Microbial community of a waste dump site. J. Appl. Sci. Environ. Mgt. Vol. 6(1) 78-8. |
| [4] | Sachin Singh (2015). Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation. ACADEMIA. |
| [5] | Yan Wang, Midori Ogawa, Kazaumasa Fukuda, Hiroshi Miyamoto, and Hatsumi Taniguchi (2006). Isolation and identification of mycobacteria from soils at an illegal dumping site and landfills in Japan. Microbiol. Immunol., 50(7), 513-524. |
| [6] | David L. Sewell, Thomas A. Golper, Peter B. Hulmau, Catharine M. Thomas, Linda M. West, Winnie Y. Kubey and Clifford J. Holmes (1990). Comparison of large volume culture to other methods for isolation of microorganisms from Dialysate. Peritoneal Dialysis International, Vol. 10, pp. 49-52. PMID: 2128189. |
| [7] | Naima Atiq, Safia Ahmed, M. Ishtiyaq Ali, Saadia Andleeb, Bashir Ahmed and Geoffrey Robson (2010). Isolation and identification of polystyrene biodegrading bacteria from soil. African journal of Microbiology. Research vol. 4(14), pp. 1537-1541. |
| [8] | D’Arcy R. Meyer-Dombarb, Jean E. Bogner and Judy Malas (2020). A review of landfill microbiology and ecology: A call for modernization with next generation technology. Front. Microbiol. 11: 1127. |
| [9] | Angel R, Claus P, Conrad R (2012). Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions. The ISME Journal 6: 847-862. |
| [10] | William Wade (2002). Unculturable bacteria-the uncharacterized organisms that cause oral infections. JR Soc Med 95: 81-83. |
| [11] | Shakti Kumar Saket (2019). Isolation and biochemical characterization of antibiotic producing microbes from soil around waste dumping sites of Rewa district. Central for biotechnology studies Awadh Pratap Singh University; Rewa (M.P.). |
| [12] | Barbara Breza-Boruta, Joanna Lemanowicz, Agata Bartkowiak (2016). Variation in biological and physiochemical parameters of the soil affected by uncontrolled landfill sites. Environ Earth Sci 75: 201. |
| [13] | Priyanka Tripathy, Pallavi Jali and Gyanranjan Mahalik (2020). Overview of isolation and identification of different bacterial and fungal species from municipal solid waste. Indian Journal of Natural Sciences Vol. 10/Issue 60. |
| [14] | Dariusz Roman Ropek (2021). Changes in bacterial soil biota under cultivation of crops near a municipal landfill site. Agronomy. 11, 2114. |
| [15] | A Iravanian, and Sh O Ravari (2020). Types of contamination in landfills and effects on the environment: A review study. IOP Conf. Ser.: Earth Environ. Sci. 614012083. |
APA Style
Singh, S., Srivastava, D. (2024). Isolation of Microbes from Landfill Soil of Gorakhpur, Uttar Pradesh, India. International Journal of Microbiology and Biotechnology, 9(3), 61-67. https://doi.org/10.11648/j.ijmb.20240903.13
ACS Style
Singh, S.; Srivastava, D. Isolation of Microbes from Landfill Soil of Gorakhpur, Uttar Pradesh, India. Int. J. Microbiol. Biotechnol. 2024, 9(3), 61-67. doi: 10.11648/j.ijmb.20240903.13
AMA Style
Singh S, Srivastava D. Isolation of Microbes from Landfill Soil of Gorakhpur, Uttar Pradesh, India. Int J Microbiol Biotechnol. 2024;9(3):61-67. doi: 10.11648/j.ijmb.20240903.13
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Download@article{10.11648/j.ijmb.20240903.13,
author = {Shalini Singh and Deepa Srivastava},
title = {Isolation of Microbes from Landfill Soil of Gorakhpur, Uttar Pradesh, India
},
journal = {International Journal of Microbiology and Biotechnology},
volume = {9},
number = {3},
pages = {61-67},
doi = {10.11648/j.ijmb.20240903.13},
url = {https://doi.org/10.11648/j.ijmb.20240903.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20240903.13},
abstract = {This study focuses on the isolation and characterization of microbes from landfill soil in Gorakhpur, India. Landfills are a major source of soil pollution, and understanding the microbial composition in these environments is crucial for assessing soil quality and developing remediation strategies. Microorganisms can degrade numerous of organic pollutants owing to their metabolic machinery and to their capacity to adapt to inhospitable environment. Thus, microorganisms are major players in site remediation. However, their efficiency depends on many factors, including the chemical nature and the concentration of pollutants, their availability to microorganisms, and the physio-chemical characteristics of the environment. The research identified a diverse microbial community in the landfill soil, with predominant bacterial representatives including Gamma-proteobacteria, firmicutes, and bacteroids. Gram staining revealed the prevalence of gram-positive bacilli, along with distinct fungal species. These findings emphasize the potential of microorganisms in degrading organic pollutants and transforming various compounds in landfill soil. By elucidating the microbial diversity in landfill sites, this study provides insights for sustainable waste management practices and environmental conservation efforts. One of the major cause of soil pollution are landfills. There are the sites designated for dumping rubbish, garbage, or other sorts of solid wastes. Because most of these waste materials are non-biodegradable, they heap in the landfills where they stay for years, impacting on soil quality and polluting the land. The aim of this study is to isolate and investigate the role of microorganisms in a particular landfill area of Gorakhpur, Uttar Pradesh and to identify the microbial community found in that particular area.
},
year = {2024}
}
TY - JOUR T1 - Isolation of Microbes from Landfill Soil of Gorakhpur, Uttar Pradesh, India AU - Shalini Singh AU - Deepa Srivastava Y1 - 2024/08/15 PY - 2024 N1 - https://doi.org/10.11648/j.ijmb.20240903.13 DO - 10.11648/j.ijmb.20240903.13 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 61 EP - 67 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20240903.13 AB - This study focuses on the isolation and characterization of microbes from landfill soil in Gorakhpur, India. Landfills are a major source of soil pollution, and understanding the microbial composition in these environments is crucial for assessing soil quality and developing remediation strategies. Microorganisms can degrade numerous of organic pollutants owing to their metabolic machinery and to their capacity to adapt to inhospitable environment. Thus, microorganisms are major players in site remediation. However, their efficiency depends on many factors, including the chemical nature and the concentration of pollutants, their availability to microorganisms, and the physio-chemical characteristics of the environment. The research identified a diverse microbial community in the landfill soil, with predominant bacterial representatives including Gamma-proteobacteria, firmicutes, and bacteroids. Gram staining revealed the prevalence of gram-positive bacilli, along with distinct fungal species. These findings emphasize the potential of microorganisms in degrading organic pollutants and transforming various compounds in landfill soil. By elucidating the microbial diversity in landfill sites, this study provides insights for sustainable waste management practices and environmental conservation efforts. One of the major cause of soil pollution are landfills. There are the sites designated for dumping rubbish, garbage, or other sorts of solid wastes. Because most of these waste materials are non-biodegradable, they heap in the landfills where they stay for years, impacting on soil quality and polluting the land. The aim of this study is to isolate and investigate the role of microorganisms in a particular landfill area of Gorakhpur, Uttar Pradesh and to identify the microbial community found in that particular area. VL - 9 IS - 3 ER -
Department of Microbiology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, India
Department of Botany, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, India
Figure 1. Pure cultures on LB Agar plates.
Figure 2. Pure culture on PDA Agar plates.
Figure 3. Showing microscopic view of Gram's stain slides of different pure colonies (A, B, C, D).
Figure 4. Showing Zone of inhibition of different antibiotics against bacterial culture.
Figure 5. Showing zone of inhibition at different concentration of Cefotaxime against bacterial culture.
